The recombinant expression systems for structure determination of eukaryotic membrane proteins
Publication Year
2014
Type
Journal Article
Abstract
Eukaryotic membrane proteins, many of which are key players in various biological processes, constitute more than half of the drug targets and represent important candidates for structural studies. In contrast to their physiological significance, only very limited number of eukaryotic membrane protein structures have been obtained due to the technical challenges in the generation of recombinant proteins. In this review, we examine the major recombinant expression systems for eukaryotic membrane proteins and compare their relative advantages and disadvantages. We also attempted to summarize the recent technical strategies in the advancement of eukaryotic membrane protein purification and crystallization.
Keywords
Journal
Protein Cell
Volume
5
Issue
9
Pages
658-72
Date Published
09/2014
ISSN Number
1674-8018
Alternate Journal
Protein Cell
PMID
25119489